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No Peak is obtainable

http://www.chromforum.org/viewtopic.php?t=6448

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No Peak is obtainable

Posted: Wed Jul 18, 2007 6:23 am
by denial
Can some one help me in solving my Problem.

I am running reverse phase HPLC methods for the extracts of Hypericum perforatum (St John Wort), and using Methanol and Phosphoric acid in gradient run. But unfortunately I am getting nothing.

Can some one tell me where I am doing mistake.

Thanks to All

Posted: Wed Jul 18, 2007 5:16 pm
by Mark Tracy
Show us a chromatogram of your "nothing" and the gradient conditions. The active component of St. John's Wort is hypericin, which is intensely colorful. I suspect an instrument problem, but without more information, I can't say more.

Posted: Fri Jul 20, 2007 6:33 am
by denial
Show us a chromatogram of your "nothing" and the gradient conditions. The active component of St. John's Wort is hypericin, which is intensely colorful. I suspect an instrument problem, but without more information, I can't say more.
You were right, there was some problem in Detector, it is now remedized.
Thanks
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