Advertisement
Chromatography Forum

Retention time(tR) and baseline peak bandwidth (Wb)

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
Topic locked

Retention time(tR) and baseline peak bandwidth (Wb)

avatar
project44
Is there any mathematical correlation between the retention time of an analyte and the baseline peak bandwidth? I want to express the Wb of the peak as peak volume (Vc). I think that this can be calculated easily, but i would like some advice of how to estimate it.Is this equation correct ? ( Vc=4Vm(1+k)/N^1/2 where Vm is the column dead volume, k is the retention factor and N the number of theoritical plates).

avatar
Uwe Neue
Your equation is correct
Topic locked
2 posts Page 1 of 1
Return to “Liquid Chromatography”

Who is online

In total there are 28 users online :: 0 registered, 0 hidden and 28 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 28 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

    Gas Chromatography

      Mass Spectrometry