Chromatography Forum

Hi, professionals,
I am working on conjugated linoleic acid analysis, who can give me some idea, how to prepare sample, and usually, how many isomers can be detected? what kind of column used?

thanks,
have a nice weekend!

As has been stated several times before, when working with Lipids,
consider two major Internet and book resources.

For formal lipid analysis methods, AOCS is the first port of call, and in this case they also publish the series " Advances in conjugated linoleic acid research ", which will probably discuss analytical issues and techniques. If you want to use GC ( which isn't the only techniques, as nmr, UV, IR and HPLC ( UV or ELSD detection ) have also been used), you may be looking at a 100 meter capillary column.

For research lipid analysis methods, W.W.Christie is the expert, and has provided a CLA summary with pointers to published information-
http://www.lipidlibrary.co.uk/topics/cla/index.htm
along with a detailed practical guide.
http://www.lipidlibrary.co.uk/topics/cla_meth/index.htm

There are a lot of analytical issues associated with CLA analysis, so a literature search should be performed to ensure the technique and conditions you select will be appropriate for your samples.

Bruce Hamilton

thank you very much!

http://www.sigmaaldrich.com/Brands/Supe ... etins.html

The above web page has a link to bulletin 907. It is a free downloadable PDF file. It may be helpful.

best wishes,

Rodney George
Senior Research and Development Scientist
Gas Separations Research
Supelco
595 North Harrison Road
Bellefonte, PA 16823

814-359-5737 voice
814-359-5459 fax
rodney.george@sial.com