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Saponins
Posted: Thu Jun 21, 2007 10:06 am
by KJM
I need to try to develop an HPLC method for the separation & identification of saponins. I know they have a poor UV chromophore & that Evaporative light scattering detector may be most suitable. Initially I only want to run on an analytical scale. If anyone has any recommendations for column type, mobile phases & gradients to use I would be very grateful.
Thanks, KJM.
Posted: Thu Jun 21, 2007 1:02 pm
by philippem
looking on google helpds a lot : for saponins and HPLC analysis gives you al lot of references
try this hyperlink:
http://www.lcgcmag.com/lcgc/data/articl ... rticle.pdf
regards
philippe
Posted: Thu Jun 21, 2007 1:32 pm
by KJM
Phillipe, yes I'd been working my way through the Google responses too. Thanks for the link, this is definitely a good method to start with.
KJM
Do a search on Ginseng
Posted: Mon Jul 16, 2007 8:49 pm
by Tony_Montanari
Do a keyword search on Ginseng. It will lead you to a list of emails in which I produced an analytical method for the measurement of Ginsenosides. These are all saponins.
I am currently working on ion-exchange resin cleanups of Ginseng extracts for better HPLC measurement.