MS-TOF

[Albert Roy J]

I am trying to identify the 3 impurities on my compound that were nicely resolved in my method on LC. My question is, when those impurities went to the MS, will the resulted chromatogram in the MS would correlate to the LC part? Meaning, if impurity A eluted at 3.5, would i see a peak in the chromatogram in the MS screen at 3.5, too? Pls. enlighten and big thanks.

[Kostas Petritis]

If your chromatographic conditions are the same then you will have almost the same retention factors (retention time may vary a little bit due to void volume differences).

Remember that you must use volatile mobile phase additives to be compatible with the MS.

Also, your compounds need to be ionized in order to be seen by your MS...