Odd chromatagrams for sugar analysis
Posted: Tue May 15, 2007 7:52 pm
I use an HPLC machine at work to measure sugars in cereals. Recently we have been getting odd chromatagrams. We always do 2 injections for each sample and while 1 injection will give a good base line with 4 peaks detected as normal another will give a small negative peak and a flat line with no peaks detected at all for the same sample. This seems to happen at random. 1 run it may not happen at all, another maybe once and another it will do it on every other injection.
We have sent the detector off to be looked at, we were told there was a leak in it and this was subsequently fixed, the lamp was also changed but this hasn't made any difference.
Can anyone throw any light onto what the problem might be and how to fix it.
We have sent the detector off to be looked at, we were told there was a leak in it and this was subsequently fixed, the lamp was also changed but this hasn't made any difference.
Can anyone throw any light onto what the problem might be and how to fix it.