Extraction method for propionic acid in bread

[Louise77]

Dear All Expert Chromtographers,

I am looking for an extraction method of propionic acid in bread. I would like to run it on a HPLC rather than GC due to their availability in my lab. Looking for any advises from you all.

Thanks and regards.

[philippem]

Dear Louise77

This is anextraction method, but analysis done by GC. Most of the literature ( try www.google.com) mention GC analysis due to nature of product and (non) detectability with UV

Gas chromatographic determination of propionic acid in bread and cake.Isshiki K, Tsumura S, Watanabe T.
A simple and accurate method is presented for gas-liquid chromatographic (GLC) determination of propionic acid in bakery products. Propionic acid is extracted with a mixture of dichloromethane and formic acid to which isobutyric acid has been added as an internal standard. The extract is injected directly into the GLC system. A glass column packed with 5% SP-1000 is used. The simplicity and convenience of the method make it suitable for routine determination.

Hopes this helps

philippe

[Uwe Neue]

There are possibilities, but they depend on the detection tools that are available to you. If you can do low-UV detection. I would suggest HILIC on silica (80% acetonitrile, 20% water on Atlantis HILIC silica). An alternative would be low UV detection with water with a small amount of phosphoric acid and an acid-stable water-compatible C18 (such as Atlantis T3). Both methods would also work with RI detection.

[Consumer Products Guy]

What about HPLC on an organic acid column with conductivity detection?