Chromatography Forum

Could anyone please tell me whether I could use dimethyl sulfoxide or dimethylformamide as sample solvent for a reversed phase HPLC method.
Many thanks in advance!

Hi Luren

This is an example of using DMF and DMSO in peptide analysis by RP HPLC

http://www.freepatentsonline.com/5932547.html

Try to find similiar articles on google
Best regards

You can use DMSO or DMF for your sample solvent, but there are a few cautions. 1. Both absorb strongly in the UV so you will get a big void peak. Any analytes near void will be affected. 2. They are very strong solvents, so beware of weak eluents or the peak shapes will be very bad. If possible, dilute the sample with water and/or minimize injection volume. 3. They are more viscous than typical HPLC solvents, so the pressure will spike up upon injection. This can be rough on column packings; some columns withstand better than others. Minimizing injection volume helps here too.

You got some good advice already from Mark above. One more thing: be careful about the possibility that a fraction of the analytes can be carried through the column with the unretained peak of DMSO or DMF. Unfortunately, this is tough to verify with UV, but it can be seen readily with MS.

Dear All,

Thank you all for your advices.

Kind regards

Luren