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- Posts: 3
- Joined: Tue Apr 03, 2007 12:32 am
Hello, I am in need of a buffer or solvent that can extract Triglycerides along with Cholesterol and HDL from dried serum. The serum was dried in a filter paper medium. I need a solvent or buffer that will allow diffusion and solubalization of all three analytes from filter paper in a dried form. I wonder if rehydration first would make extraction easier? Also, It does not matter if other serum substances are extracted along with the Cholesterol and triglycerides and HDL lipoproteins. I was thinking about a buffer solution such as PIPES with detergent for an example which would allow diffusion of the three anlalytes however I do not know if hydrophobic Cholesterol or the lipids in the triglyceride portion will diffuse away from a paper solid phase? I know that some solvents work ok such as methanol. I do know that ethanol will not work because it interferes with detection of the triglycerides bye the detection method that I will use where methanol does not effect the detection but its extraction efficiency is lower then methanol. I know that cholesterol is very soluble in acetone, but I do not know what it would do to the fatty triglyceride or the protein portion of the HDL (high density lipoprotein). Any suggestions or comments would be appreciated. The extraction has to be a simple one and I can’t use two or three different extractions as it should be one for all three. Also, it is for screaning , so I cant do any HPLC on the screaning samples. Regular analysis by a clinical analyzer would be used to confirm abnormal screens and it will also be used to estimate the three analytes
Basically, I need a one step buffer or solvent that will rehydrate and solubilize serum serum cholesterol and HDL and triglycerides. . Thanks so much.. Mark
Basically, I need a one step buffer or solvent that will rehydrate and solubilize serum serum cholesterol and HDL and triglycerides. . Thanks so much.. Mark
