Chromatography Forum

Hi everybody!

Could somebody please tell me if it is the pressure drop in the column which causes the high pump pressure in hplc or if the high pressure is used to achieve good separation results (for better adsorption or something like that) ? Do i need to build a special tube behind the column for the eluent to relax?

Thx


Cheers
Chris

let me tell you something my dear friend
HPLC=High pressure liquid chromatography
that high pressure is becouse you using 3-5 µ column to get resolution as smaller the partical size more the surface area available for your analyte n mobile phase interact.

i hope You understood HPLC Basic Funadamental

Could somebody please tell me if it is the pressure drop in the column which causes the high pump pressure in hplc or if the high pressure is used to achieve good separation results

It's the pressure drop across the column. The "high performance" comes from using small particles. The "high pressure" comes from having to maintain a constant flow through a column tightly packed with those small particles.

Other interpretations of "HPLC" include:
"high priced" LC
"highly perplexing"
"high patience" LC
and "hocus pocus" LC
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