separation of 2 methyl butyricacid and 3 methyl butyric acid

[annethebault]

i want to separate these acids in HPLC;. Is it possible with column H+ (87HPX Biorad), in which conditions? If not, what kind of column is available. ThankYou

[AA]

Will you pay me (or any other poster) to do your job for you? Try some research, do some reading. Do your job. Then, if you are still have trouble, perhaps you could post again with some detail what you have tried so far.

[annethebault]

Will you pay me (or any other poster) to do your job for you? Try some research, do some reading. Do your job. Then, if you are still have trouble, perhaps you could post again with some detail what you have tried so far.

Thank you very much for your help!!! I have not time to answer at this stupid game!!!

[pharmason]

there is no way one can answer your question.
no such information is there in question. i would say question is in air.

[Alfred88]

Dear all:
I thought that all the people in France are elegant and polite. But today I learn that I am wrong (or perhaps only annethebault has not progressed beyond his adolescent!).

Any one monitoring this forum should have seen excellent posts by Bruce Hamilton about method development. The "gold standard" is trying it out with a short (15 cm) C18 column, with gradient MeCN.
Another solution is to out-source this task to a contract lab (cost $10K).

Alfred.

[tom jupille]

Wow, looks like people had a rough weekend.

annethebault: AA was blunt in his comment, but his point is valid: in general, the posts that get the most useful responses here on the Forum are the ones where the poster has done some preliminary work or study and asks specific questions.

Probably the first place to look would be on the Bio-Rad web site to see what they have in the way of applications information. Check with other column manufacturers to see if they have anything relevant.

All that said, Alfred88's suggestion is a good one (using a low-pH buffer) with Acetonitrile. That separation should be straightforward by standard reversed-phase .

[AA]

I apologize for being so blunt, but I really get annoyed when people are too lazy to do a simple web search or flip through the literature, or in this a quick phone call to Bio-Rad.

I don’t know about most of the posters to this board, but I get paid to solve problems, create separations, develop methods and similar activities, and I find the best place to start in the library (we call ours the "Information Center") and rather than just doing, I learn why thing happen and learn how I can make (or not make) them happen in my lab.

Too few of us are willing to learn and keep learning.

Again, my apologies if I offended anyone.

AA

[annethebault]

I apologize for being so blunt, but I really get annoyed when people are too lazy to do a simple web search or flip through the literature, or in this a quick phone call to Bio-Rad.

I don’t know about most of the posters to this board, but I get paid to solve problems, create separations, develop methods and similar activities, and I find the best place to start in the library (we call ours the "Information Center") and rather than just doing, I learn why thing happen and learn how I can make (or not make) them happen in my lab.

Too few of us are willing to learn and keep learning.

Again, my apologies if I offended anyone.
In fact, i am french and my english is very bad. So, its not easy for me to explain what i had made before . Effectively, i did some researchs before but i have no time to do more because i am a technical controller and machines are not available to do these because of production's controls. But i am perplex when i read your first answer.


AA