Missing Peaks

[syx]

I changed the outlet tubing of autosampler with new one and all peaks were missing though the flow was ok. Could a new tubing absorbed the analytes?

[Consumer Products Guy]

I doubt it, my guess is some kind of leak. One question is why did you change the tubing, and second is did you change anything else there as well. If neither of those, inject some 10x concentration and see what happens then.

[syx]

... the sample loop was disconnected by someone or 'something'. problem has been solved.