Best wayt to profile lipids
Posted: Thu Apr 26, 2007 11:35 am
I have to profile fatty acids and no-saponificable lipids such as sterols, etc.. Agilent has offered to me two options:
1) Using a HP-88 (highly polar) column of 100m.
Provides cis-trans separation of fatty acids ( I need that!!) but nobody knows how the behavior of sterols and another lipids in this column.
2) Using a 3 column system.
Using a short DB-5 (higly apolar) column in the start of chromatography. Next a flow spliter system to pass the fraction of fatty acids ( from 0 to 10 min) to a DB-23 system that provides cis trans separation. The another part from the short DB-5 is passed to a much longer DB-5 that provides a good separation of sterols and another
Which one do you recommend me??
Or do you have another suggestion???
1) Using a HP-88 (highly polar) column of 100m.
Provides cis-trans separation of fatty acids ( I need that!!) but nobody knows how the behavior of sterols and another lipids in this column.
2) Using a 3 column system.
Using a short DB-5 (higly apolar) column in the start of chromatography. Next a flow spliter system to pass the fraction of fatty acids ( from 0 to 10 min) to a DB-23 system that provides cis trans separation. The another part from the short DB-5 is passed to a much longer DB-5 that provides a good separation of sterols and another
Which one do you recommend me??
Or do you have another suggestion???