Purity of anhydride by HPLC

[lotussss]

Hello,

I try do determinate purity of an anhydide by hplc without using derivatization.

Thanks.

[Mark Tracy]

Since anhydrides react with water, alcohols, amines, etc. you should think about normal-phase chromatography. Even then, many common stationary phases adsorb water or have alcohol functional groups.

Are you sure you don't want to derivatize?

[avitan]

May be Normal Phase HPLC, using aprotic solvents (MtBE/Hexane) and water-control procedures for stationary phase?

(search the forum for analysis of water sensitive materials)