Peaks in the blank---Back to the forum(Help needed)

[LC_Trouble]

Hi everyone,

Thanks for all the replies.I tried all your suggestions but unfortunately i am still getting the peaks betwenn 15-25 mins.I have to get rid of these peaks because its going to affect my samples.The final step i read from the articles suggests that i can clean up the filter with the nitric acid.It would be of great help to me if somebody have a previous expereince in cleaning up the filter..Can you please suggest me like what concentration of nitic acid should i use and also the procedure of cleaning up.

Thanks.

[lpnian]

if you use gradient elution in your expeiment,it will couse the gost peak if water and your agent is dirty

[DR]

Search my posts using terms gradient and Empore. You will find some suggestions for proper water clean-up.