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- Posts: 4
- Joined: Mon Apr 16, 2007 8:42 pm
Is it possible to have a detector that detects ONLY the signals from the solute molecules residing in the mobile phase? I presume that techniques like fluorescence will respond to solute molecules irrespective of it resides in the column packing solid or the mobile phase flowing through its interstices.
Because of this, I am intrigued why band broadening theories base their calculations on the mobile phase concentration distribution, say as in the book called Dynamics of Chromatography written by Giddings. Thanks in advance.