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Purity of anhydride by HPLC
Posted: Mon Apr 09, 2007 3:14 pm
by lotussss
Hello,
I try do determinate purity of an anhydide by hplc without using derivatization.
Thanks.
Posted: Mon Apr 09, 2007 3:49 pm
by Mark Tracy
Since anhydrides react with water, alcohols, amines, etc. you should think about normal-phase chromatography. Even then, many common stationary phases adsorb water or have alcohol functional groups.
Are you sure you don't want to derivatize?
Posted: Thu Apr 19, 2007 4:36 pm
by avitan
May be Normal Phase HPLC, using aprotic solvents (MtBE/Hexane) and water-control procedures for stationary phase?
(search the forum for analysis of water sensitive materials)