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FPLC

Posted: Wed Mar 28, 2007 12:58 pm
by Hana
The molecule that I am trying to purify/isolate has a molecular weight of 450.
I would like to know what type of desalting column is recomended to use.

Posted: Wed Mar 28, 2007 4:02 pm
by Uwe Neue
You could do this with a column packed with Oasis HLB, if your analyte is hydrophobic. Is it retained on a reversed-phase column? How much do you need to desalt?