Chromatography Forum

Dear members,
In method development sometimes we need to change used wavelength. For example, in first time of development we used 254 nm and we did method validation, DAD was used for this purpose. In the middle of validation process we found that we should change the wavelenght. Instead of repeat all steps of validation those had been done, we want to re-process the data. Could anyone give us the guidance about how to perform re-process or re-integrate using different wavelength on Agilent Chemstation? It is possible to perform?

Thanks and regards,
Siswanto

If you acquired the DAD data in the mode where you recorded/saved the spectra, every 2nd spectum, etc., then you can change the wavelength in the DAD set up, set up your integration and calibration at that wavelength, then save the Method and reprocess the data. However, if you only acquired the 254 nm signal, you are SOL. That's an American term for "too bad". A reason one doesn't acquire additional spectral data all the time is that the files are larger.