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- Posts: 4
- Joined: Mon Aug 07, 2006 7:25 am
Hello all, I was hoping that someone may be able to steer me in the right direction as to the following query.
The company I work for are a pharmaceutical company that primarily produce amino acid mixtures containing a few excipients and flavours. Currently our amino quantification is performed via reverse phase HPLC using a uv/vis detector. For the amino acids such as taurine that show no response to such a detection a derivitization procedure ( usually with fluorecamine ) is carried out prior to injection. We have recently began to do some of our quantification via an ELSD detector and I was after some information as to where to look for resources on methods that may assist in the detection of amino acids using this detector.
There are three primary reasons for the move to the ELSD detector as outlined below
* The larger responses gained will allow more opportunity to accurately quantify our more complex amino acid mixtures ( up to 12 amino acids per mixture )
* There will be increased opportunity to homogenise our methods thus decreasing preparation time due to machine equilibration
* The detection of amino acids currently unable to be quantified without derivitisation will be possible.
I truly would appreciate any assistance in our move to the ELSD detector and look forwards to hearing from you shortly
Regards,
Peter
The company I work for are a pharmaceutical company that primarily produce amino acid mixtures containing a few excipients and flavours. Currently our amino quantification is performed via reverse phase HPLC using a uv/vis detector. For the amino acids such as taurine that show no response to such a detection a derivitization procedure ( usually with fluorecamine ) is carried out prior to injection. We have recently began to do some of our quantification via an ELSD detector and I was after some information as to where to look for resources on methods that may assist in the detection of amino acids using this detector.
There are three primary reasons for the move to the ELSD detector as outlined below
* The larger responses gained will allow more opportunity to accurately quantify our more complex amino acid mixtures ( up to 12 amino acids per mixture )
* There will be increased opportunity to homogenise our methods thus decreasing preparation time due to machine equilibration
* The detection of amino acids currently unable to be quantified without derivitisation will be possible.
I truly would appreciate any assistance in our move to the ELSD detector and look forwards to hearing from you shortly
Regards,
Peter
