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Methionine containing peptid separation?

Posted: Wed Mar 14, 2007 6:55 pm
by noam
Hello all,

I was wondering if some one could help me out,
I need to separate 2 small proteins, one containing a Met group and one missing it.

I think I remember something about a Sephadex modification that might have good interactions with the Met group but I’m not sure if it's commercially available from GE or not.
If any one have ideas that could help, it would be great.

regards, noam

Posted: Thu Mar 15, 2007 7:41 pm
by Kostas Petritis
There is a Methionine enrichement kit sold by PIERCE (I think) that might be good for your purposes. The specificity is very good, however the yields are not as good...

thank you

Posted: Fri Mar 16, 2007 6:07 am
by noam
Thank you,
I would check it out
noam