hi all

[raghunandan]

can any one help me in the method development of reltaed substance for escitalopram

[Rob Burgess]

Its a bit hard to know where to start with this one... you haven't given us much info to go on... (even in the title of your message) try "Googling" it!

[raghunandan]

can any one help me in the method development of ESCITALOPRAM OXALTE RELATED SUBSTANCE

[raghunandan]

PLEASE LET ME KNOW WHAT INFORMATION U WANT
TAHNKS IN ADVANCE

[JA]

Hi and welcome to the board. Please check the following useful links:

Please read this
Where to look first!
How do you do method development?

[SIELC_Tech]

You probably can use any reverse phase column for this compound:
http://en.wikipedia.org/wiki/Escitalopram

Some of the related impurities can be hydrophilic and not UV active (N,N-dimethylisopropyl fragments), so you might want to use LC/MS first to make sure that you see your impurities.

ACN/water/buffer (phosphate or sulphate for UV) with gradient of ACN from 5% to 70%. For LC/MS use formic acid or ammonium formate with the same gradient. Watch for peak shape due to residual silanol interaction with your amine.