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Void volume marker for SAX phase

Posted: Fri Mar 09, 2007 11:45 am
by martin lund
What are the most common void volume markers for anion exchange columns? The compound should be UV/Vis detectable.

Thanks

Posted: Fri Mar 09, 2007 1:18 pm
by danko
Hi Martin,

Anny UV absorbing, neutral compound should do the trick. Acetone might be a good choice.

Best Regards

Posted: Fri Mar 09, 2007 5:50 pm
by Bruce Hamilton
If your AX column is resin-based, you may find that solvents dissolve into it, so it may be preferable to use a slightly higher MW neutral compound.

Bruce Hamilton