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Bethanechol Chloride by Conductivity Detection

http://www.chromforum.org/viewtopic.php?t=5468

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Bethanechol Chloride by Conductivity Detection

Posted: Fri Feb 16, 2007 5:13 am
by ErikaMac
Hi,

Does anyone have any experience with this analysis? I have been running the USP monograph, Bethanechol Chloride related compounds, and am experiencing real difficulties in achieving the required sensitivity.
I have tested the anaysis on several detectors (Shimadzu CDD-10A, CDD-6A, Waters 430 and 431) and in all cases I fail to detect the required 0.1ug/mL level.

All conditions are run as per the monograph and it is in unsuppressed mode.

Thanks

Posted: Sun Feb 18, 2007 4:57 am
by Uwe Neue
Are the retention times and the column volume in agreement with the USP criteria?

Bethanechol Chloride by Conductivity Detection

Posted: Sun Feb 18, 2007 10:52 pm
by ErikaMac
The retention times and column volume are consistent with the USP criteria. (RRT 2-HPTA 0.93, Column Vol ~1.2mL)
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