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starting of hplc method development

Posted: Tue Feb 06, 2007 2:00 pm
by don_111
if api contain two acid group then how i start hplc method development

Posted: Tue Feb 06, 2007 2:39 pm
by Consumer Products Guy
acidic mobile phase....does API have UV absorbance.....what's API soluble in....

Posted: Tue Feb 06, 2007 2:55 pm
by Peter Apps
literature search for method for similar molecule

Posted: Tue Feb 06, 2007 4:42 pm
by SIELC_Tech
Check this step by step guide based on the properties of your compound. It will ask you bunch of questions about nature of your compound and will give you advice:
http://www.sielc.com/MethodDevelopment_ ... dType.html

Here is couple others (based on properties and your detection technique):

http://www.sielc.com/MethodDevelopment_Guide.html
http://www.sielc.com/Request_Literature.html

Contact me if you have any questions

Vlad