starting of hplc method development

[don_111]

if api contain two acid group then how i start hplc method development

[Consumer Products Guy]

acidic mobile phase....does API have UV absorbance.....what's API soluble in....

[Peter Apps]

literature search for method for similar molecule

[SIELC_Tech]

Check this step by step guide based on the properties of your compound. It will ask you bunch of questions about nature of your compound and will give you advice:
http://www.sielc.com/MethodDevelopment_ ... dType.html

Here is couple others (based on properties and your detection technique):

http://www.sielc.com/MethodDevelopment_Guide.html
http://www.sielc.com/Request_Literature.html

Contact me if you have any questions

Vlad