honey analysis

[vismaior]

Hi everybody,

I start HPLC and GC work with honey samples now. Anybody has experience about sample preparations, methods, anything else???

thanks,
best regards,

vismaior

[Consumer Products Guy]

HPLC with refractive index or ELSD detector would likely be best single overall choice. GC can be used on the smaller components (mono- and di- saccharides), which are usually made into acetate or trimethylsilyl derivatives.

[Mary Carson]

It all depends on what you plan to accomplish with your analyses. Provide some more details, please.

[vismaior]

Thaks for answers.

I would like to analyse theese:
- sulfonamides,
- streptomycin,
- chloramphenicol,
- tyosin,
- amitraz,
- brompropylat,
- coumaphos,
- fumagillin,
- naphtalin,
- nitrofuranes.

I have already experiences about fenol, sugars and tetracyclines.

I have HPLC (Merck-Hitachi) with UV, FL, and RI detectors, and Agilent GC with Micro-ECD and FID detectors.

thanks, vismaior

[Fabiano]

I already did in meat:

- sulfonamides, piece of cake:
http://md1.csa.com/partners/viewrecord. ... cookie=yes


- chloramphenicol, the only positive I saw in brazilian meat.
http://cat.inist.fr/?aModele=afficheN&cpsidt=16638050


-streptomycin, is like Ivermectin also requires derivatization
http://www.atypon-link.com/AOAC/doi/abs ... 04.87.1.39


find methods for the other ones should be easy.

Best regards
Fabiano

[vismaior]

Thanks a lot for informations !

best regards,
vismaior

[Mary Carson]

I believe most of the nitrofuran monitoring methods out there use a mass spectrometer. A mass spec is also useful for the compounds on your list that don't have a chromophore, like streptomycin.