Chromatography Forum

Has anyone got any literature methods for the analysis of phospholipids (especially phosphatidylserine) from oils by HPLC or GC.

Thanks

If you're working with lipids, you should obtain a copy of W.W.Christie's book Lipid Analysis, currently in third edition, publication details are available at his website, along with many free general sections on analysis, including PLs.

http://www.lipidlibrary.co.uk/topics/hplc_pl/index.htm

A Google search will also provide many methods, but W.W. Christie's book has been through several revisions, and the methods are usually robust.

Bruce Hamilton

Thanks. Got plenty of HPLC parameters but has anyone got methods for extraction from oils?

Christie; chapter 3

Might find some useful information here..

http://www.avantilipids.com/TechnicalInformation.html