Splitless purge flow HS-SPME
Posted: Wed Nov 22, 2017 9:25 am
Hello everybody,
I am running a few GC/MS SPME headspace methods in Splitless mode. I have a fiber conditioning station, so I do not need to condition the fiber in the inlet post-run. For example I let the fiber in the inlet for 2 minutes and then I move it to the conditioning station.
I have some questions regarding the "Purge flow to split valve" parameter. I can set the flow (default is 50 ml/min) and the minutes.
If I understood well, after X minutes, a helium flow of X ml/min is sent to the split valve. This is supposed to empty the septum from residuals solvents and compounds (tell me if I am wrong).
1° Do I need to purge the inlet with a high flow even if I am using a solvent-less techinique like HS-SPME ?
2° Now I have set it to 50 ml/min after 2 minutes. Do you think it makes sense to reduce the flow to save gas? (15 ml/min which is equal to my gas saving schedule)
Thank you very much
I am running a few GC/MS SPME headspace methods in Splitless mode. I have a fiber conditioning station, so I do not need to condition the fiber in the inlet post-run. For example I let the fiber in the inlet for 2 minutes and then I move it to the conditioning station.
I have some questions regarding the "Purge flow to split valve" parameter. I can set the flow (default is 50 ml/min) and the minutes.
If I understood well, after X minutes, a helium flow of X ml/min is sent to the split valve. This is supposed to empty the septum from residuals solvents and compounds (tell me if I am wrong).
1° Do I need to purge the inlet with a high flow even if I am using a solvent-less techinique like HS-SPME ?
2° Now I have set it to 50 ml/min after 2 minutes. Do you think it makes sense to reduce the flow to save gas? (15 ml/min which is equal to my gas saving schedule)
Thank you very much