Chromatography Forum

Dose anyone know the brand of HPLC column which can bear high concentration of salt without reducing of lifetime.

HIC (hydrophobic interaction chromatography) columns, for example, are designed to be used with 4N sodium sulfate, and most ion exchange columns can used with >1N sodium chloride.

Yes, I'm being sarcastic.

That question is unanswerable without more information like:
- mode of chromatography ?
- intended pH ?
- what salt ?
- organic solvent ? which one? how much?
- are you referring to salt in the mobile phase or in the sample?

Let us assume that you are talking about reversed-phase chromatography: for these columns, a high salt concentration in the mobile phase does not affect lifetime (assuming pH is within the allowed range for the column). If you are talking about injecting a high salt concetration on such columns, the problem is probably a different one: precipitation of salt. No column likes such a thing...

Tom is right: your question needs to be clearer...

I think Neue and tom is right.
As you can use high salt in revrse phase only in specified pH and morever you can give washing to column extensively as you will finish your work. If you will give washing first 100% water followed by solvent ratio in which your generally storing column.