[req] Condition for C18 column for Vitamin C Tablets, thanks

[kingsemma]

Can anyone tell me the condition and mobile phase for Vit C Tablets using C18 column (Waters Symmetry) and PDA detector?


Thank you very much!

[skunked_once]

Have you tried a literature search or contacting the vendors for application notes?

[Mark Tracy]

Vitamin C is so hydrophilic that it elutes in plain phosphate buffer at pH 3.0 +/- 0.5. The optimum pH depends on the sample matrix and interferences that you want to avoid. The Symmetry column is probably not the best option; a polar-embedded column is the better option. UV detection at 246; the spectrum is rather distinctive. Do you need to distinguish iso-ascorbic? If so, you need a long column.

[SIELC_Tech]

Here is a nice alternative using mixed mode approach:

http://www.sielc.com/compound_149.html

You can achieve reasonable retention (5-15 min on 150 mm column) by playing with mobile phase pH and ion strength.

Contact me if you have questions

Vlad

[Bryan Evans]

If you want to analyze ascorbic acid without adding ion pairing reagents,
you can try using a silica column. This separation was done on
our Unison UK-Silica column:

http://www.imtakt.com/TecInfo/TI154E.pdf