Chromatography Forum

Hi everybody,

I start HPLC and GC work with honey samples now. Anybody has experience about sample preparations, methods, anything else???

thanks,
best regards,

vismaior

HPLC with refractive index or ELSD detector would likely be best single overall choice. GC can be used on the smaller components (mono- and di- saccharides), which are usually made into acetate or trimethylsilyl derivatives.

It all depends on what you plan to accomplish with your analyses. Provide some more details, please.

Thaks for answers.

I would like to analyse theese:
- sulfonamides,
- streptomycin,
- chloramphenicol,
- tyosin,
- amitraz,
- brompropylat,
- coumaphos,
- fumagillin,
- naphtalin,
- nitrofuranes.

I have already experiences about fenol, sugars and tetracyclines.

I have HPLC (Merck-Hitachi) with UV, FL, and RI detectors, and Agilent GC with Micro-ECD and FID detectors.

thanks, vismaior

I already did in meat:

- sulfonamides, piece of cake:
http://md1.csa.com/partners/viewrecord. ... cookie=yes


- chloramphenicol, the only positive I saw in brazilian meat.
http://cat.inist.fr/?aModele=afficheN&cpsidt=16638050


-streptomycin, is like Ivermectin also requires derivatization
http://www.atypon-link.com/AOAC/doi/abs ... 04.87.1.39


find methods for the other ones should be easy.

Best regards
Fabiano

Thanks a lot for informations !

best regards,
vismaior

I believe most of the nitrofuran monitoring methods out there use a mass spectrometer. A mass spec is also useful for the compounds on your list that don't have a chromophore, like streptomycin.