UPLC

[Alejandro]

UPLC is not a good option of chromatograpy, you cannot inject dirty samples, what is daily in enviromental analisys, so that "NO SIRVE PARA NADA"...

[danko]

Hi Alejandro,

Depends on what you mean by dirty samples. If you mean particle containing liquids, you’re right – you can’t and you shouldn’t inject those. But the rule is equally true, regardless the system you’re operating.
Surely UPLC can’t be blamed for shortcutting a filtration step, which obviously is mandatory in your type of analysis.

Best Regards

[AA]

Alejandro

You have made a very strong, negative statement, which is not really true. As a matter of fact, separations on small particles at high linear velocities (that is basically what UPLC is) is a very powerful separation technique that can provide many benefits. Faster run times, better resolution, increased sensitivity, higher plate count (or peak capacity with gradients) to name the big ones. Is UPLC the answer to every chromatographic problem? Of course not, and, as a previous poster noted "dirty samples", regardless of the technique used are generally a bad thing. I would suggest you try and educate yourself a little bit before spitting fire next time.

AA

[Uwe Neue]

I am not sure if it is ever a good idea to inject a sample that is known to be "dirty". I killed my very first column with a single injection of milk, and another one shortly thereafter with a well dissolved sample of tar. Since that time (close to 30 years ago), I know that sample pretreatment is not a bad idea...

[jvjkorn]

Alejandro

Conozco muchos clientes en mexico de waters que han devuelto los sistemas de UPLC a fabrica. Una solución es el sistema RRLC 1200 de Agilent que puede trabajar tanto en columnas sub2 um y columnas trdicionales. Y si es verdad a que te refieres con muestras sucias talvez requieras mayor filtración de la muestra

[danko]

Hi Alejandro,

If you don’t care whether or not the rest of the board understand your message, why bother posting it?

[danko]

I’m sorry. The previous post should be addressed to jvjkorn

[jtreacy]

I was bored doing my 3 year business plan so translated the Spanish note above using AltaVista translations because my Spanish extends to ordering a beer... I hope I haven't misrepresented the guy

"I know many Waters clients in Mexico who have given back their UPLC systems to the manufacturers. A solution is the 1200 system from Agilent that can work equally well with sub 2um columns and conventional columns.

And Filter your samples "

John

[Newman768]

@jvjkorn, @Alejandro: *plonk*