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Rolling collision Energy

http://www.chromforum.org/viewtopic.php?t=50634

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Rolling collision Energy

Posted: Sun Oct 15, 2017 10:44 am
by Frabbani
Hi everybody,
In "Rolling collision Energy", how are the equations (used to define the m/z vs. CE relationships) determined for a peptide mixture? Should the separate experiment be used for each peptide in peptide mixture?
Thanks in advance,
Faride

Re: Rolling collision Energy

Posted: Fri Oct 27, 2017 2:20 am
by JMB
Isn’t the RCE procedure meant to avoid the need for optimizing colln energy for each and every peptide?
Surely you set up system to acquire scans across each chromatographic peak using a program that increases the colln energy in steps across a pre-set range.

Regards,
JMB
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