Rolling collision Energy

[Frabbani]

Hi everybody,
In "Rolling collision Energy", how are the equations (used to define the m/z vs. CE relationships) determined for a peptide mixture? Should the separate experiment be used for each peptide in peptide mixture?
Thanks in advance,
Faride

[JMB]

Isn’t the RCE procedure meant to avoid the need for optimizing colln energy for each and every peptide?
Surely you set up system to acquire scans across each chromatographic peak using a program that increases the colln energy in steps across a pre-set range.

Regards,
JMB