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IP reagents + buffers
Posted: Wed Nov 22, 2006 6:22 am
by Philip
Can anyone tell me what the effect of IP reagents (e.g. alkylsulfonates) and buffers (e.g. Ammonium Acetate/Glacial Acetic Acid) + organic, mixed, might be...? Suppose it might be a better question to ask if there is any adverse or potential positive system mixing IP reagents + buffer...?
Thanks alot!
Regards,
Phil
Posted: Wed Nov 22, 2006 2:45 pm
by danko
Hi Philip,
No problem, as long as the plan is thought through. By that I mean: You should ensure that your analyte is positively charged while the alkylsulfonate is negatively charged. So start with finding the pKa for these and adjust the buffer accordingly. Remember, the buffer pH should be adjusted in accordance with its buffer capacity range, i.e. pKa ± 1. Acetate’s pKa is 4.75.
Good luck
Re: IP reagents + buffers
Posted: Wed Nov 22, 2006 3:14 pm
by 4ugirish
Can anyone tell me what the effect of IP reagents (e.g. alkylsulfonates) and buffers (e.g. Ammonium Acetate/Glacial Acetic Acid) + organic, mixed, might be...? Suppose it might be a better question to ask if there is any adverse or potential positive system mixing IP reagents + buffer...?
Thanks alot!
Regards,
Phil
Dear philip I am not clear about your question, kindly elaborate it
Posted: Sun Nov 26, 2006 8:02 pm
by tom jupille
Make sure that everything is soluble when mixed (good idea to try mixing typical proportions in a test tube to check).
Posted: Mon Nov 27, 2006 4:27 pm
by Dan
Phil,
As an example of what Tom wrote: don't use the potassium salt of a buffer reagent if the IP reagent is SDS. You will get a precipitate.
Regards,
Dan
Posted: Wed Dec 13, 2006 5:24 am
by ym3142
As an example of what Tom wrote: don't use the potassium salt of a buffer reagent if the IP reagent is SDS. You will get a precipitate.
Dan, I donot understand your claim since I have used KH2PO4 and SDS all the time. Thanks for explaination