Early eluted peak
Posted: Fri Sep 22, 2017 4:42 pm
I'm using 1200 Agilent HPLC with Max-RP column (2.00 x 50) 4 (µm).
mobile phase is 5mM Ammonium formate in 10% methanol and 5mM Ammonium formate in 90% methanol.
My analyte retention time is 0.5 min with 0.5 mL/min flow rate.
the dead volume is 0.1 mL.
I want the peak to elute little bit later than 0.5 min.
I tried to reduce the flow rate to 0.25 ml/min but it showed a border peak.
I'm thinking to increase the buffer concentration in my mobile phase, what other solutions do you suggest that might help me to get a later RT.
mobile phase is 5mM Ammonium formate in 10% methanol and 5mM Ammonium formate in 90% methanol.
My analyte retention time is 0.5 min with 0.5 mL/min flow rate.
the dead volume is 0.1 mL.
I want the peak to elute little bit later than 0.5 min.
I tried to reduce the flow rate to 0.25 ml/min but it showed a border peak.
I'm thinking to increase the buffer concentration in my mobile phase, what other solutions do you suggest that might help me to get a later RT.