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HPLC: Problems with pressure

http://www.chromforum.org/viewtopic.php?t=49604

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HPLC: Problems with pressure

Posted: Fri Sep 22, 2017 3:54 pm
by adolfoc
the pump is constantly triggered by pressure, what should I check? Should I clean it with isopropanol? the flowpath will it be blocked?
please help me as soon as possible. Thanks in advance.

Re: HPLC: Problems with pressure

Posted: Fri Sep 22, 2017 4:44 pm
by HPLC chemist
It could be several things like the pressure trigger is set too low! Usually 5000 psi is enough to prevent a C18 or DVSB column from being crushed.

Above 10,000 psi the nuts and bolts start flying across the room (LOL)!
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