solubility of drug in sesame oil
Posted: Sat Sep 16, 2017 5:34 pm
Dear everyone,
Could you please suggest for my case?I would like to determine the solubility profile of my drug in sesame oil. After 3 days shaking and centrifugation, I get the supernatant and then mixed with the organic solvent (MeOH) to inject into HPLC.The problem is sesame oil can't mix with MeOH.
After that, I try to mix with the co-solvent system, Iso-propyl alcohol-tetrahydrofuran, and MeOH. Sesame oil is miscible with this solvent mixture. Before injecting into HPLC, I checked with UV and it showed that absorbance of blank is very high and negative absorbances of the sample also. I think it has interference.
I would like to analyze by HPLC but I worry for my column because sample contains oil and tetrahydrofuran.
I use mobile phase of 63:37 organic phase (ACN and MeOH) and aqueous phase (Water and Phosphate buffer)
Column is C18 Hypersil gold
Please, could someone suggest or advice for my case? Could I inject this sample into my column or how can I solve this problem by UV?Hope your kind reply.Thanks so much.
The main thing is miscibility of sesame oil with the organic solvent to inject into HPLC.
Could you please suggest for my case?I would like to determine the solubility profile of my drug in sesame oil. After 3 days shaking and centrifugation, I get the supernatant and then mixed with the organic solvent (MeOH) to inject into HPLC.The problem is sesame oil can't mix with MeOH.
After that, I try to mix with the co-solvent system, Iso-propyl alcohol-tetrahydrofuran, and MeOH. Sesame oil is miscible with this solvent mixture. Before injecting into HPLC, I checked with UV and it showed that absorbance of blank is very high and negative absorbances of the sample also. I think it has interference.
I would like to analyze by HPLC but I worry for my column because sample contains oil and tetrahydrofuran.
I use mobile phase of 63:37 organic phase (ACN and MeOH) and aqueous phase (Water and Phosphate buffer)
Column is C18 Hypersil gold
Please, could someone suggest or advice for my case? Could I inject this sample into my column or how can I solve this problem by UV?Hope your kind reply.Thanks so much.
The main thing is miscibility of sesame oil with the organic solvent to inject into HPLC.