Chromatography Forum

Posted: **Thu Nov 02, 2006 5:08 pm**

Dear Peter , Bruce and all

In on column injection people inject large vlume of solvent such as 10 ul do they need to keep the volume of standard same during calibration?

please send me some explanation please !!!!!!!!!!!!!!!!!11111

Posted: **Thu Nov 02, 2006 5:43 pm**

I would - that's 1 less source of variability to worry about.

Posted: **Thu Nov 02, 2006 6:02 pm**

I totally agree.

best wishes,

Rod

Posted: **Mon Nov 06, 2006 1:12 pm**

In general, standards and samples must be treated in eactly the same way, which includes injection volume.

As an aside, on-column injections are not necessarily of large volumes - if large volumes are used you get all sorts of solvent effects that add extra complications to method development.

Peter

Chromatography Forum

question regarding on column injection

question regarding on column injection

on column injection