question regarding on column injection

[rajdeeproy]

Dear Peter , Bruce and all

In on column injection people inject large vlume of solvent such as 10 ul do they need to keep the volume of standard same during calibration?

please send me some explanation please !!!!!!!!!!!!!!!!!11111

[DR]

I would - that's 1 less source of variability to worry about.

[chromatographer1]

I totally agree.

best wishes,

Rod

[Peter Apps]

In general, standards and samples must be treated in eactly the same way, which includes injection volume.

As an aside, on-column injections are not necessarily of large volumes - if large volumes are used you get all sorts of solvent effects that add extra complications to method development.

Peter