Page 1 of 1

Ammonium bicarbonate vs 4-Methylmorpholine as lcms buffer

Posted: Thu Aug 17, 2017 6:24 pm
by Aldijkhuizen
I would like to compare ammonium bicarbonaat buffer vs 4-Methylmorpholine for buffering at ph 8. Can i lower the ph of 100 mM ammonium bicarbonaat with formic acid? What acid/base do you use to adjust ph?

Re: Ammonium bicarbonate vs 4-Methylmorpholine as lcms buffer

Posted: Thu Aug 17, 2017 9:19 pm
by sav
I would like to compare ammonium bicarbonaat buffer vs 4-Methylmorpholine for buffering at ph 8.
The pH stability of ammonium bicarbonate buffer is not good (water must be degassed before buffer preparation). The good practise is preparation of fresh AB buffer before application.
Can i lower the ph of 100 mM ammonium bicarbonaat with formic acid?
Yes

Re: Ammonium bicarbonate vs 4-Methylmorpholine as lcms buffer

Posted: Fri Aug 18, 2017 2:21 pm
by Aldijkhuizen
by he way, what acid/base do i use o adjust pH of 4-methylmorpholine?

Re: Ammonium bicarbonate vs 4-Methylmorpholine as lcms buffer

Posted: Sun Aug 20, 2017 1:53 pm
by sav
by he way, what acid/base do i use o adjust pH of 4-methylmorpholine?
formic acid/ammonium hydroxide