Contact Closure Problem of Waters UPLC and Thermo Orbitrap
Posted: Wed Aug 09, 2017 5:11 pm
Hi,
We just installed a new Waters Acquity UPLC and connected it to a Thermo LTQ-Orbitrap. The issue is that sometime mass spec could not be triggered by UPLC., which means the gradient has already started but XCalibur still shows mass spec is "Waiting for contact closure". Sometime that happened at the beginning of a sequence. Sometime mass spec failed to be triggered in the middle of a sequence and the sequence would be blocked. Does anyone know what is the reason of that? How to prove if the contact closure device of UPLC is working fine or just broken? This issue really disturbed me a lot. Thanks a lot.
We just installed a new Waters Acquity UPLC and connected it to a Thermo LTQ-Orbitrap. The issue is that sometime mass spec could not be triggered by UPLC., which means the gradient has already started but XCalibur still shows mass spec is "Waiting for contact closure". Sometime that happened at the beginning of a sequence. Sometime mass spec failed to be triggered in the middle of a sequence and the sequence would be blocked. Does anyone know what is the reason of that? How to prove if the contact closure device of UPLC is working fine or just broken? This issue really disturbed me a lot. Thanks a lot.