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RID vs ECD for Sugar analysis

Posted: Wed Aug 02, 2017 4:41 am
by MyaqM
Hello,

I am trying to decide on either an RI or EC detector for sugar analysis. Does anyone have experience with either of these?

Thanks in advance.

Re: RID vs ECD for Sugar analysis

Posted: Wed Aug 02, 2017 10:25 am
by HPLC chemist
I have routinely used an RID detector for simple sugar analysis per ICUMSA
(International Commission of Uniform Methods of Sugar Analysis) guidelines. The RID is 'very' prone to interferences (alright for HFCS, jams, jelly beans), it is insensitive (it detects sub%), temperature or pressure changes...

Re: RID vs ECD for Sugar analysis

Posted: Thu Aug 03, 2017 8:56 pm
by Jake
This recent thread discusses RID and sugars. Maybe some info of use to you there:

viewtopic.php?f=1&t=44761

Re: RID vs ECD for Sugar analysis

Posted: Sun Aug 13, 2017 12:04 pm
by sav
I am trying to decide on either an RI or EC detector for sugar analysis.
1. Relatively high concentration of sugars, relatively simple matrix of sample and isocratic conditions (only) = RID

2. Otherwise = ECD

Re: RID vs ECD for Sugar analysis

Posted: Sun Aug 13, 2017 12:55 pm
by MyaqM
Many thanks everyone.

I guess ECD is the way to go.

Thanks Jake for the link :)