Chromolith
Posted: Tue Oct 10, 2006 10:26 am
by sridharkam
Has anyone tried the merck chromolith column for separation of water soluble vitamins? if yess is there a significant improvement over conventional columns
Monolithes
Posted: Tue Oct 10, 2006 8:21 pm
by Chris0000
I did hear many happy users of monolihes
The general benefit is a lower backpressure and the resulting faster runtime, about 5 times faster than packed columns.
I cant´t say If it works for yor vitamin application, but if it´s normal RP ( -18 ) mode, it should work.
regards Chris
Posted: Wed Oct 11, 2006 8:40 am
by vicentexxx
hi sridharkam,
i don't know why you want to use a chromolith to separate water soluble vitamins. In effects tha main goal with this type of column is a smaller time analysis but if you want different selectivity i don't think it's a good choice. Moreover if you are interested in vitamin B1 B3 B6 there are a lot of methods with short time analysis. But i don't really know your intents and expectations...that is if the core of your experiment is column or separation itself...
Here's a link with some HPLC methods
http://www.discoverysciences.com/chroma ... =&keyword=
Posted: Sun Oct 15, 2006 6:04 am
by malaka50