Chromatography Forum

Dear all,
Does anybody know any Reverse phase LC method for DPPE?
DPPE is not soluble in water, acetone, MeOH, EtOH.
I find it has certain solubility in Propylene Glycol and IPA.
I am trying to avoid normal phase LC method at current stage.
Thanks to all.

Assuming DPPE is 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine,
then the following paper is freely available on the WWW.

They used a C8 column with a ternary solvent system, dichloromethane (phase A), methanol (phase B), and a 30 mM TBABr aqueous solution (phase C). The proportion of phase C was maintained at 15% during all experiments, resulting in a constant concentration of 4.5 mM TBABr.

Bacterial phospholipid molecular species analysis by ion-pair reversed-phase HPLC/ESI/MS
Nicolas Mazzella*, Josiane Molinet*, Agung Dhamar Syakti*, Alain Dodi, Pierre Doumenq1,*, Jacques Artaud* and Jean-Claude Bertrand
Journal of Lipid Research, Vol. 45, 1355-1363, July 2004

Good luck,

Bruce Hamilton