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Hemoglobin A2 seperation

http://www.chromforum.org/viewtopic.php?t=4644

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Hemoglobin A2 seperation

Posted: Fri Sep 22, 2006 10:36 am
by hadye
Hi guys,

I am still novice in HPLC and I am trying to seperate hemoglobin variants on conventional HPLC, I am facing problem in separating Hb A from A2. I am using Cation exchange colum with pH gradient. I have tried pH 6.4 & 6.1 but thereis no seperation

Can anyone help me
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