Hb A2 seperation
Posted: Thu Sep 21, 2006 9:28 am
Hello everybody,
I am new member of this forum and still novice in HPLC, I am trying to seperate different Hb types on HPLC 1100 series. The problem that I have is that I cannot seperate HB A from A2.
I am using a cation exchange column 2.5ux100mm. I tried using phoshate buffer and tris for pH but still I cannot seperate A2. All the peaks whether A S F C can be seperated but always A2 is hidden in the A tail in chromatogram
Can anyone help me in this issue
I am new member of this forum and still novice in HPLC, I am trying to seperate different Hb types on HPLC 1100 series. The problem that I have is that I cannot seperate HB A from A2.
I am using a cation exchange column 2.5ux100mm. I tried using phoshate buffer and tris for pH but still I cannot seperate A2. All the peaks whether A S F C can be seperated but always A2 is hidden in the A tail in chromatogram
Can anyone help me in this issue