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serching equivalent column
Posted: Wed Sep 20, 2006 8:26 am
by vicentexxx
hi everyone,
i' m replacing an analytical method in which a column XTerra RP18, 150*4.96, 5 micron is used.
i haven't got it so, could someone device me what column to choose?
Thank you averyone
Posted: Wed Sep 20, 2006 1:04 pm
by Bryan Evans
Depends on what your experimental conditions are
(i.e. mobile phase composition, column temp., ect).
Posted: Wed Sep 20, 2006 3:00 pm
by vicentexxx
condition are:
flow: 0.8 ml/min
mobile phase: MeOH/ACN/buffer pH 3 perchlorate 35/17/48
detector: DAD
inj volume: 30 mcL
i know Xterra is a column with both silica and polymer components, but i have only typical C18 column (Alltima, Luna, LichroCart etc and polar column)...
Posted: Wed Sep 20, 2006 3:02 pm
by tom jupille
Posted: Thu Sep 21, 2006 2:28 pm
by vicentexxx
Hi tom ,
Thank you very much, i think the database you send me will be very useful.
Vincenzo