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diluted collagen by HPLC

http://www.chromforum.org/viewtopic.php?t=46151

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diluted collagen by HPLC

Posted: Fri Jun 30, 2017 9:16 pm
by hplcnewuser1
Hi, I have a solution of collagen: 0.05mg/ml in 1/1 solution of PBS/diwater.
How can I show a collagen peak by HPLC?
Any help appreaciated

Re: diluted collagen by HPLC

Posted: Fri Jun 30, 2017 10:03 pm
by HPLC chemist
Collagen like all proteins absorb at 214 nm (along with everything else). You can review this article;

http://www.acgpubs.org/JCM/2015/Volume% ... 5-01-2.pdf
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